Using selective reagents in the separation technique of complexation counter-current chromatography can improve the resolution effectively.The commonly used selective reagents include metal ion selective reagent,polysaccharideion selective reagent,anionic surface active agent,ion pair selective reagent,chiral reagent and acid-base reagents of pH-zone-refining.In this paper,the applications of different kinds of selective reagents in counter-current chromatography were summarized.The fundamental principle was introduced,and the way to improve the resolution was analyzed.Finally,the scope of application and the development trend of each selective reagents were also discussed.