Illegal drugs added in the process of livestock production would seriously affect the food safety in our country.The residues of 3 β2-agonists（e.g.，clenbuterol，salbutamol and ractopamine）in animal urine were prepared with deuterated isotope internal standard QuEChERS method ,and analyzed by gas chromatography-mass spectrometry（GC-MS）.Samples were enzymaticed with β-glucuronidase，and extracted with ammoniation ethyl acetate，then cleansed with anhydrous MgSO4 and primary secondary amine（PSA）sorbent.After supernatant was dried and derivatized，the analysis of target compounds was performed with GC-MS.The results indicated that good linear relationships were obtained for three β2-agonists in the certain concentration with correlation coefficients -greater than 0.998.The average recoveries of 3 analytes at three spiked concentration levels ranged from 95.0% to 101.0%，with relative standard deviations（RSDs）of 2.5%-6.2%.The limits of detection（LOD） were in the range of 0.3-0.6 μg/L and the limits of quantitation（LOQ） were 1.0-2.0 μg/L.This method has the advantages of simplicity，rapidness，stable operation，high precision，and is suitable for the determination of three kinds of β2-agonists residues in animal urine.