The residue of organophosphorus pesticide(OPPs)is one of the most serious problems of food safety in China.It is urgently necessary to determinate the OPPs in foods.However,foods are a kind of complex matrix,and the OPPs must be extracted and purified by pretreatment method.In this work,the novel pretreatment methods of OPPs for instrumental analysis,including solid phase extraction,liquid-liquid microextraction,ultrasonic extraction,microwave assisted extraction,immune chromatography and enrichment by nano-materials etc,were introduced.The principles,advantages,disadvantages and the applications of these methods were introduced.This review provides a reference for related research projects.