Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System

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Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System

更新时间：2023-05-18

- Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System
- Vol. 34, Issue 5, Pages: 595-599(2015)
- 作者机构：
  
  福建医科大学药学院药物分析系
- 作者简介：
- 基金信息：
- DOI：
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Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System. [J]. 34(5):595-599(2015)
DOI：

Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System. [J]. 34(5):595-599(2015) DOI：

摘要

亚硫酸氢钠（NaHSO3）和过氧化氢（H2O2）反应可产生微弱的化学发光。核黄素对亚硫酸氢钠和过氧化氢的化学发光有极大的增强作用，且化学发光强度在0.02~2.0 μg/mL范围内与核黄素浓度呈良好的线性关系。据此，建立了核黄素的高灵敏化学发光分析方法，方法检出限为0.007 μg/mL，相对标准偏差（n=11）不大于1.6%。利用荧光光谱、化学发光谱对核黄素增强NaHSO3-H2O2体系化学发光的机理进行探讨，为该体系的应用研究提供了新思路。

Abstract

The reaction between NaHSO3 and H2O2 produced ultra-weak chemiluminescence(CL).Riboflavin could greatly enhance the CL from NaHSO3-H2O2 system.The CL intensity was linearly increased with concentration of riboflavin in the range of 0.02-2.0 μg/mL.Based on the phenomenon,a high sensitive chemiluminescent method was developed for the determination of riboflavin in tablet samples.The detection limit was 0.007 μg/mL and the relative standard deviation was not more than 1.6%.Furthermore,fluorescent and chemiluminescent spectrum was utilized to illustrate the chemiluminescent mechanism,which would extend the application of NaHSO3-H2O2 system.

关键词

化学发光亚硫酸氢钠过氧化氢核黄素

Keywords

chemiluminescencesodium bisulfitehydrogen peroxideriboflavin

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陕西师范大学化学与化工学院

西安科技大学化学与化工学院

Jiangsu Key Laboratory of Environmental Science and Engineering

School of Environmental Science and Engineering，Suzhou University of Science and Technology

Center of Materials Science and Optoelectronics Engineering， University of Chinese Academy of Sciences

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