An innovative analytical approach based on the use of comprehensive two-dimensional hydrophilic interaction/reversed phase liquid chromatography（HILIC/RPLC） was applied to obtain the profiling of phenolic compounds in Chuju.The method involved the use of a microbore hydrophilic interaction column containing a diol stationary phase（Lichrospher diol-5） in the first dimension combined with a reversed phase column（Cortecs C18+） in the second dimension，and applied two sample loops as interface between the first and the second dimension.Two different gradient elutions were employed in the two dimensions，respectively.Detecton wavelength was set at 330 nm,and sampling rate was 15 Hz in the diode array detection.The theoretical peak capacity was up to 1 117 while the surface coverage proposed by Gilar（SCG） was 0.56，indicating both good resolution and good orthogonality of this 2D-LC system.23 peaks in the 2D-LC separation space were found，and 13（apigenin，luteolin，acacetin，acacetin-7-O-glucoside，chlorogenic acid，isorhamnetin-3-O-glucoside，luteolin-7-O-glucoside，acacetin-7-O-rutinoside，acacetin-7-O-rutinoside，quercitrin，isochlorogenic acid A，isochlorogenic acid B，apioside）of them were allowed tentative identification according to comparing with retention times and maximum absorption wavelengths between the sample and standards.In which 4 phenolic compounds（isorhamnetin-3-O-glucoside，acacetin-7-O-rutinoside，eriodictyol-7-O-glucoside，apioside）were firstly found in Chuju and isorhamnetin-3-O-glucoside was firstly found in Dendranthema morifolium.