New molecularly imprinted polymers（MIPs） were prepared by using attapulgite（ATP） as the support,β-CD and methacrylic acid（MAA） as functional monomer,resveratrol as template molecule through surface molecularly imprinted polymerization technology.The MIPs were used as sorbent filled into the multiplug filtration clean-up（m-PFC） column for resveratrol enrichment and purification.A new method was developed for the determination of resveratrol by using multiplug filtration clean-up column with molecularly imprinted polymers extraction（MIPs-m-PFCE） combined with fluorescence spectrometry.The results showed that the present method has a high selectivity for RES under the optimized experimental conditions,the adsorption capacity of MIPs was 12.78 mg/g.The calibration curve was linear in the range of 0.1-100 μg/mL with a correlation coefficient（r） of 0.999 7.The limit of detection was 0.048 μg/L.The proposed method was successfully applied in the determination of RES in peanut root,with average recoveries of 86.2%-102.1% and relative standard deviations（RSDs） of 3.5%-5.8% at three spiked levels.The developed method is rapid and selective,and is adaptable to the analysis of trace RES in real samples.