A method of HPLC-quadrupole/electrostatic field orbitrap HRMS（HPLC-QE HRMS）was established to analyze 10 polyphenols in wine with the help of qualitative confirmation of HRMS.The samples were extracted with ethyl acetate after acidized.The qualitative confirmation was carried out by full scan and target MS2 spectra after separation on an ACQUITY UPLC BEH C18 column（2.1 mm×100 mm,1.7 μm）.An external standard method was used for quantitative analysis.The limits of quantitation（LOQs） of 10 polyphenols ranged from 0.0005 mg/L to 0.0025 mg/L.The spiked recoveries were between 82.0% and 109.1% with RSDs less than 10%.