Cancer antigen-153(CA-153) was very significant specific tumor marker.An Au nanoparticles/graphene oxide(Au NPs/GO)-based electrochemical immunosensing platform was fabricated for the sensitive detection of CA-153 by the sandwich immunoassay protocol.Au NPs/GO composite material was used to modify the glassy carbon electrode.CA-153 antibody was anchored on the surface of Au NPs by the absorbtion of Au NPs and CA-153 antibody.Bovine Serum Albumin(BSA) was used to block possible remaining active sites of nanomaterials and avoid the nonspecific adsorption.AuAg cube nanomaterial available to catalyze the reduction of hydrogen peroxide was used to lable antibody(Ab2).The electrochemical signals derived from the carried AuAg cube toward the reduction of H2O2 using the thionine as electron mediator,and were measured by differential pulse voltammetry.Under the optimized experimental conditions,the linear range and the detection limit(S/N=3) of CA-153 immunosensor were 2.0×10-5-100 U/mL and 7.0×10-6 U/mL,respectively.The proposed method was applied in the detection of CA-153 in spiked human serum samples with recoveries of 92.2%-110.2% and RSD not more than 8.7%.