Based on high performance liquid chromatography combined with time of flight mass spectrometry(HPLC-TOF MS),the metabolomics method was established to evaluate the urine samples collected from 30 farmers,an occupational exposure population in Zhouwang village,Shanxi province,P.R.China.These people were exposed to a circumstance with acetamiprid,lambda-cyhalothrin,bifenthrin acetate and methyl amino abamectin benzoate.The urine samples were detected before the exposure,and at the first day,the third day,the fifth and seventh day after the exposure. Extracting 10 common metabolites in urine and quality control samples showed that the method has high precision and reliability.Principal component analysis(PCA) score plot was used to separate the urinary metabolic profiles from the different time urine sample,and the results showed that the urine metabolic profiles before and after the exposure to pesticide were obvious different.36 different ions were found by PCA loading plot model,in which 8 ions were identified by retrieving metabolites database and identified with standard.The most statistically significant changes were observed for dopamine,serotonin,tyrosine,tryptophan,taurine and hippuric acid(downward trend),canine urea and creatine(upward trend).This work suggests that an exposure to pesticide mixtures induces metabolism disturbance.It is inferred that the pesticide mixtures could lead to tryptophan metabolism intermediates decreased,accumulation of hepatic and energy metabolism change may related with the nervous system and liver function damage.These changes of metabolites existed in nervous and liver system are consistent with the mechanism of four kinds of pesticides which could cause damage to insecticidal nervous system.