An HPLC-MS/MS methord was developed for the simultaneous determination of 14 forbidden colorants,including metanil yellow,acid violet 49,solvent red 49,solvent blue 35,tetramethylpararosaniline chloride(TMPC),pentamethyltriphenylmethane(PMTPE),hexamethylenepara rosaniline chloride(HMPC),pigment orange 5,pigment red 53,sudan I,sudanⅡ,Sudan Ⅳ,rhodamine B,disperse yellow 3,in cosmetics.Cosmetic samples were extracted and dispersed with tetrahydrofuran,and some matrixes were precipitated out after addition of ammonium acetate solution and methanol.After centrifuged and filtered,the extract was separated on an Agilent poroshell 120 EC-C18 column(2.7 μm,3.0 mm×50 mm)by gradient elution with 10 mmol/L ammonium acetate-acetonitrile as mobile phase.The quantitative analysis was performed by the standard curve method of peak area.The linear ranges of 14 analytes were in the range of 0.01-1.0 μg/mL,with correlation coefficients more than 0.999.The limits of quantitation(LOQs)were in the range of 0.05-0.5 μg/g.The average recoveries at three spiked levels ranged from 85.4% to 106.3% with RSDs less than 10%.This method is accurate,simple,sensitive and reliable,and could be used for the analysis of 14 forbidden colorants in cosmetics.
Simultaneous Determination of 14 Forbidden Colorants in Cosmetics by High Performance Liquid Chromatography
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大连市产品质量监督检验所
Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,),Guangzhou
Guangzhou Institute for Drug Control
Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology,Guangdong Provincial Engineering Research Center for Quality and Safety of Traditional Chinese Medicine
Guangdong Institute for Drug Control,National Medical Products Administration Key Laboratory for Safety Risk Assessment of Cosmetics