In this study,tannic acid(TA) functionalized graphene nano sheets(TA-G) was facilely synthesized through a one-step green reduction method using tannic acid as dual function agent，in which tannic acid acted as not only a reduction reagent to reduce oxided graphene(EGO) but also a capping reagent to functionalize graphene.The as prepared TA-G was used to construct a graphene based rutin electrochemical sensor,and the modified electrode had a high sensitivity for rutin detection.The results of electrode reaction kinetics of rutin on TA-G modified electeochemical sensor indicated that the electeochemical reaction of rutin on the modified electrode undergone a surface controlled quasi reversible electrochemical process.The optimal conditions were as follows:pH value:3.0,TA-G amount(1.0 μg/mL) on TA-G modified electrochemical sensor:8 μL,scan rate:100 mV/s,cycles of enrichment:100.Under the optimal conditions,the fabricated sensor displayed an excellent analytical performance for rutin detection,with a linear range of 1.0×10-8-1.0×10-5 mol?L-1 and a low detection limit of 6.0×10-9 mol?L-1 at 3σ.Meanwhile,the fabricated rutin electrochemical sensor also exhibited good selectivity,specificity and stability,and could be used for rutin detection in real samples.The proposed method broadened the application field of graphene and offered a new idea for rapid detection of drug.