A method for the simultaneous determination of 5 plant growth regulator residues,including 6-benzyl adenine（6-BA），thidiazuron，forchlorfenuron，paclobutrazol and uniconazole in fruits was developed by using ultra high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） with solid phase extraction（SPE）.Samples were extracted with acetonitrile，then the extracts were concentrated and cleaned up on a NH2 solid phase extraction cartridge with dichloromethane-methanol（92∶8） as elution solution.Sample preparation was accomplished after concentrated and redissoved.The UPLC-MS/MS method was performed with Waters ACQUITY UPLC BEH C18（50 mm×2.1 mm，1.7 μm） column using water-methanol as mobile phase by gradient elution，at a flow rate of 0.3 mL/min.The target compounds were determined by UPLC-MS/MS under multi reaction monitoring（MRM） mode，and then quantitatively analyzed by external standard method using the matrix-matched standard solution.The results showed that there were good linearities for 5 plant growth regulators between peak areas and concentrations in the range of 5-500 ng/mL under the optimal conditions，with correlation coefficients（r2） of 0.996 1-0.999 6.The spiked recoveries of 5 plant growth regulators in fruits at three spiked levels of 0.004，0.02，0.1 mg/kg ranged from 75.6% to 110.5% with relative standard deviations（RSDs） of 1.2%-12.8%.The limits of detection of this method（LOD） were in the range of 0.001-0.002 mg/kg，and the limits of quantitation（LOQ） were 0.003-0.006 mg/kg.The established method was simple，sensitive，accurate and reliable，and could meet the requirements of multi pesticide residues analysis in fruits.