A method was developed for the simultaneous determination and identification of ribavirin and amantadine residues in poultry meat by liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were purified with solid phase extraction column of PCX and QuEChERS method after extraction with 1% trichloroacetic acid-methanol(1∶1),then separated on a BEH HILIC column(2.1 mm×100 mm,1.7 μm) by gradient elution with mobile phases of 5 mmol/L ammonium acetate-methanol.An internal standard method was used for quantitative analysis.The electrospray was operated in positive mode and a multiple reaction monitoring(MRM) as survey scan.The results showed that the calibration curves were linear in the range of 0.50-200 μg/L for two analytes with correlation coefficients(r) more than 0.997.The limits of detection(LODs,S/N ≥ 3) for ribavirin and amantadine were 0.30 μg/kg and 0.15 μg/kg,respectively,and the limits of quantitation(LOQs,S/N ≥ 10) were 1.00 μg/kg and 0.50 μg/kg,respectively.On the basis of LOQs,the intra-day average recoveries for two analytes in poultry meat samples at three spiked levels(1,2,10 times LOQ) ranged from 92.5% to 104.7% with relative standard deviations(RSDs) of 3.4%-7.8%,while the inter-day average recoveries ranged from 93.3% to 106.0% with RSDs of 6.0%-10.7%.The method was sensitive,convenient and accurate,and could satisfy the demands for detection of ribavirin and amantadine residues in poulrty meat.