Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS

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Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS

- Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS
- Vol. 35, Issue 9, Pages: 1132-1136(2016)
- 作者机构：
  
  上海化工研究院上海稳定性同位素工程技术研究中心
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Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS. [J]. 35(9):1132-1136(2016)
DOI：

Determination of 15N Labeled Arginine Fermentation Broth and Isotope Abundance by GC-MS. [J]. 35(9):1132-1136(2016) DOI：

摘要

建立了精氨酸、赖氨酸、丝氨酸、缬氨酸、亮氨酸等15种氨基酸的气相色谱-质谱联用（GC-MS）检测方法。以硅烷化试剂N-甲基-N-（三甲基硅烷）三氟乙酰胺（MSTFA）为衍生化试剂对氨基酸进行衍生化，在优化的色谱-质谱条件下，15种氨基酸均达到基线分离。结合混合阳离子（MCX）固相萃取柱对氨基酸发酵液实际样品进行净化，将所建立的方法用于15N标记精氨酸发酵液中的氨基酸组成（包括亮氨酸-15N、脯氨酸-15N、精氨酸-15N4、谷氨酰胺-15N和赖氨酸-15N2）分析，并根据EI图谱的离子碎片信息对15N标记精氨酸发酵液中的氨基酸同位素丰度进行计算。

Abstract

A method for the determination of arginine，lysine，serine，valine and other eleven amino acids was developed by MSTFA derivatization combined with gas chromatography-mass spectrometry（GC-MS），and fifteen amino acids reached the baseline separation.The arginine fermentation broth was purified by cationic exchange mixed-solid phase extraction（SPE）， and then detected successfully by the developed method.Finally the 15N isotope abundance was calculated by characteristic fragment.

关键词

气相色谱-质谱联用（GC-MS）衍生化氨基酸15N标记氨基酸同位素丰度

Keywords

gas chromatography-mass spectrometry（GC-MS）derivatizationamino acids15N labeled amino acidsisotope abundance

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