A method was developed for the simultaneous determination of 18 phenols pollutants in water samples by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) combined with direct injection.The water samples were filtered through 0.22 μm of polytetrafluoroethylene(PTFE) microfiltration membrane.With methanol-0.01 mol/L ammonium formate/ formic acid aqueous solution (pH 4.0) as mobile phase，the separation was performed on a Thermo Hypersil ODS column(100 mm×2.1 mm，5.0 μm) by gradient elution.The flow rate was 0.2 mL/min.The column temperature was 30 ℃.Samples were detected in multiple reaction monitoring (MRM) mode with negative electric spray ionization.The phenols pollutants were quantified by the external standard method.The calibration curves of the phenols pollutants showed good linearities in a certin concentration range with correlation coefficients(r2) not less than 0.999 1，the detection limits of phenols pollutants ranged from 0.02 μg/L to 0.51 μg/L.The relative standard deviations(RSD，n=6) of 18 phenols pollutants were in the range of 0.6%-4.3%.The spiked recoveries of 18 phenols pollutants at low,medium and high levels ranged from 84.5% to 112%.The method was sensitive，rapid and little interference.It is suitable for the simultaneous determination of 18 phenols pollutants in relatively clean water samples，such as groundwater and surface water.