A method of extraction of belsoline and eldeline in Delphinium anthriscifolium Hance was developed by pressurized microwave assisted extraction(PMAE).The single factor test and orthogonal test were used to optimize the experimental conditions.The optimum extraction conditions were as follows:particle size:100 mesh,ratio of solid to liquid:1∶60,microwave temperature:80 ℃,microwave power:560 W,microwave time:10 min.Under the optimal conditions,belsoline and eldeline were separated,and determined by HPLC using methanol-0.2% triethylamine(45∶55) as mobile phase.The linear ranges of belsoline and eldeline were in the ranges of 0.50-50.0 mg/L and 0.30 -30.0 mg/L,respectively.The recoveries of belsoline and eldeline were in the range of 98.3%-104.5%,and RSDs were 2.0% and 2.2%,respectively.Compared with the solvent reflux extraction method(SRE) ,the proposed method was simple,and showed high extraction efficiencies.