Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco(NicotianaTabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry

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Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco(NicotianaTabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry

- Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco(NicotianaTabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry
- Vol. 35, Issue 8, Pages: 968-973(2016)
- 作者机构：
  
  1. 贵州大学农学院
  2. 中国科学院南京土壤研究所土壤环境与污染修复重点实验室
  3. 铜仁学院材料与化学工程学院
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Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco(NicotianaTabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry. [J]. 35(8):968-973(2016)
DOI：

Determination of 16 Polycyclic Aromatic Hydrocarbons in Tobacco(NicotianaTabacum L.) by Four-channel Chromatograph Purification Coupled with Gas Chromatography-Mass Spectrometry. [J]. 35(8):968-973(2016) DOI：

摘要

建立了同时检测新鲜烟叶中16种多环芳烃(PAHs)的四通道色谱分离仪净化/气相色谱-质谱(GC-MS)分析方法。样品经二氯甲烷索氏提取，四通道色谱分离仪净化，GC-MS选择离子监测模式(SIM)测定和外标法定量，重点考察了新鲜烟叶提取液的分离净化条件。结果表明：16种PAHs类化合物在10~1 000 μg/kg范围内定量离子的峰面积与质量浓度呈较好的线性关系(r＞0.99)。仪器检出限(LOD)为0.35~1.75 μg/kg，方法检出限(MDLs)为1.01~2.87 μg/kg。在10,50，200 μg/kg 3个浓度水平下的加标回收率为81.0%~107.8%，相对标准偏差(RSD，n=6)为2.7%~8.0%。该方法简便、快捷、可操作性强、应用范围广，适合于烟草等作物中PAHs类化合物的分析检测。

Abstract

A method for the simultaneous determination of 16 polycyclic aromatic hydrocarbons(PAHs) in tobacco by four-channel chromatograph purification coupled with gas chromatography-mass spectrometry(GC-MS) was developed.The tobacco samples were extracted by the method of soxhlet extraction with dichloromethane,and the extract was cleaned with four-channel purification chromatograph.The eluent were determined by GC-MS in SIM mode,and quantitated by a method of external standard.The procedure of separation and purification for fresh tobacco extraction was optimized.The results indicated the calibration curves of 16 PAHs had good linear relationships in the range of 10-1 000 μg/kg(r＞0.99).The detection of limits(LODs) and method detection limits(MDLs) were 0.35-1.75 μg/kg and 1.01-2.87 μg/kg,respectively.The recoveries at three spiked concentration levels were in the range of 81.0%-107.8%with relative standard deviations(RSD,n=6) of 2.7%-8.0%.The method is suitable for the determination of PAHs in tobacco or other crops with easy operation,rapidness,high repeatability and strong practicability.

关键词

四通道色谱分离仪气相色谱-质谱多环芳烃净化烟叶

Keywords

four-channel purification chromatographgas chromatography-mass spectrometry(GC-MS)polycyclic aromatic hydrocarbons(PAHs)purificationtobacco

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