A high performance liquid chromatographic(HPLC) method was developed for the determination of 5 resin acids,including abietic acid,neoabietic acid,dehydroabietic acid,palustric acid and levopimaric acid in food contact adhesives.The conditions of sample pretreatment and chromatographic separation were optimized.The sample was dissolved with ethyl acetate,and the polymer was precipitated with methanol.After filtration with filter membrane,the extract was separated on a Venusil MP C18(2) column with acetonitrile-0.4%acetic acid(80∶20) as mobile phase at a flow rate of 1.0 mL/min,detected accurately by HPLC with diode-array detector(DAD) at 240 nm for abietic acid and neoabietic acid,270 nm for palustricacid and levopimaric acid,208 nm for dehydroabietic acid,and quantified by the external standard method.The HPLC method showed a good linearity above two orders of magnitude,with detection limits of 2.5-10.0 mg/kg.The RSDs for 5 resin acids were no more than 3.4%,and the recoveries were in the range of 92.2%-103.6%.The method has the advantages of simple operation,rapid analysis and less interference factors,and could provide credible analysis for 5 resin acidsin food contact adhesives.
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