A high performance liquid chromatographic(HPLC) method was developed for the rapid determination of five synthetic sweeteners，including acesulfame K(AK)，saccharin sodium(SA)，aspartame(ASP)，alitame(ALT) and neotame(NTM) in white spirit，mixed liquor and wine.The HPLC separation was carried out on a C18 column.Several parameters，including the composition and gradient elution program of the mobile phase，column temperature and optimum detection wavelength of diode array detector(DAD) were optimized for improving the chromatographic performance and the sensitivity of determination.The results demonstrated that the separation could be completed within 15 min by gradient elution with acetonitrile and 0.02 mol/L ammonium sulfate solution(pH 4.4) as mobile phase.The column temperature was set at 40 ℃.The calibration curves of five synthetic sweeteners were linear in the range of 4-200 mg/L with correlation coefficients more than 0.999.The recoveries were in the range of 95.2%-103.2%，with relative standard deviations(n=5) not more than 3.4%.The experimental results indicated that the established method was simple，efficient and sensitive，and was suitable for the determination of five synthetic sweeteners in white spirit，mixed liquor and wine.