A method of ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the determination of cyenopyrafen residues in fruits and vegetables.The analytes were extracted from the samples using acetonitrile,and purified by dispersion solid phase extration using C18 and PSA as solid phase,then analyzed by UPLC-MS/MS.The matrix-induced weakening effect was observed in the analysis of cyenopyrafen by UPLC-MS/MS in serval samples including of cucumber,apple and onion.The interference of matrix was reduced by the matrix-matched calibration standards curve.The linear ranges of cyenopyrafen in different matrices ranged from 0.05μg/L to 10μg/L with correlation coefficients not less than 0.9996.The recoveries of cyenopyrafen in cucumber,grape,apple and scallion samples at spiked levels of 0.00005-2mg/kg were in the range of 81.1%-99.3%,with relative standard deviations(RSDs) of 4.7%-9.3%.The limit of quantitation was 0.043μg/kg and the limit of detection was 0.013μg/kg.