Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA

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Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA

- Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA
- Vol. 36, Issue 1, Pages: 101-105(2017)
- 作者机构：
  
  1. 郑州大学化学与分子工程学院
  2. 河南工程学院材料与化学工程学院
- 作者简介：
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Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA. [J]. 36(1):101-105(2017)
DOI：

Studies on Interaction of Tyr-Tyr-Tyr-Tyr with ct-DNA. [J]. 36(1):101-105(2017) DOI：

摘要

采用Fmoc固相合成法合成了酪氨酸四肽（YYYY），经液相色谱-高效液相色谱(RP-HPLC)纯化，并用电喷雾质谱(ESI-MS)表征后，得到纯度大于90%的四肽，利用紫外光谱、圆二色光谱和荧光光谱研究了肽YYYY与ct-DNA的相互作用。结果表明，随着肽浓度的增加，ct-DNA的紫外吸收产生明显的增色效应，荧光猝灭显著，圆二色光谱发生红移。研究推测酪氨酸四肽以沟槽方式与ct-DNA发生相互作用，且酪氨酸四肽与ct-DNA的作用强于酪氨酸。

Abstract

A model peptide,Tyr-Tyr-Tyr-Tyr（YYYY）,was synthesized by Fmoc solid-phase methods,purified and separated by reverse phase high performance liquid chromatography（RP-HPLC）,and characterized by ESI-MS analysis.The interaction of peptide with calf thymus DNA(ct-DNA) was investigated by UV-Vis absorption spectra,circular dichroism(CD) and fluorescence spectra.The results showed that,with the increase of peptide concentration,there were a hyperchromic effect on UV spectra,a remarkable fluorescence quenching and a red shift of CD.It is possible that Tyr-Tyr-Tyr-Tyr and Tyr might interact with ct-DNA via the groove binding mode.The interaction between Tyr-Tyr-Tyr-Tyr and ct-DNA,compared with that between Tyr and ct-DNA,is more stronger.

关键词

酪氨酸四肽ct-DNA相互作用Fmoc固相合成法

Keywords

Tyr-Tyr-Tyr-Tyrct-DNAinteractionFmoc solid-phase methods

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Related Institution

Key Laboratory of Luminescence and Real-time Analysis of the Ministry of Education，School of Chemistry and Chemical Engineering，Southwest University

湖南省农业科学院湖南农产品加工研究所

桂林理工大学化学与生物工程学院

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