A gas chromatography-tandem mass spectrometric(GC-MS/MS) method was developed for the determination of 6 plant growth regulators(PGRs),including 4-CPA,2,4-D,NAA,IAA,2,4-D butyl ester and IBA in bean sprout and tomato.The sample was firstly extracted with acided acetonitrile.The extract was concentrated and redissovled with methanol.And then, the solution was methyl esterified with 14% boron trifluoride methanol solution.The esterified solution was extracted with ethyl acetate-hexane(2∶8,by volume) after adding 2,4-D butyl ester solution.The treated extract was determined by GC-MS/MS under multiple reaction monitoring(MRM) mode,and quantified by the external standard method.The limits of quantitation(S/N>10) of 6 PGRs in bean sprout and tomato were 15μg/kg.The recoveries of PGRs in bean sprout and tomato at three spiked levels of 15,30,60μg/kg ranged from 70.0% to 127% with relative standard deviations(RSDs) of 4.1%-11.4%.The developed method is easy, fast and accurate,and could be applied in routine test of plant growth regulators in bean sprout and tomato samples.