Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry

您当前的位置：

首页 >

文章列表页 >

Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry

- Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry
- Vol. 36, Issue 6, Pages: 809-811(2017)
- 作者机构：
  
  1. 国家烟草质量监督检验中心
  2. ．中国科学院合肥物质科学院应用技术所
  3. 河南省肿瘤医院
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry. [J]. 36(6):809-811(2017)
DOI：

Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry. [J]. 36(6):809-811(2017) DOI：

摘要

该文建立了检测尿液中泛酸含量的液相色谱-串联质谱(HPLC-MS/MS)方法，尿液经过离心、稀释后，采用ACPUITY UPLC SS T3(2.1 mm×100 mm，1.8 μm)色谱柱进行分离，电喷雾正离子模式电离，多反应监测模式进行检测，方法的线性关系良好(r=0.999 3)，方法检出限为0.46 ng/mL，回收率为87.9%~95.3%，相对标准偏差(RSD)为2.5%~13.0%。该方法具有灵敏度高、分析时间短等特点，可用于尿液中泛酸含量的分析。

Abstract

A liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm) column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient(r) of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9%-95.3%,and the RSDs are in the range of 2.5%-13.0%.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine.

关键词

液相色谱-串联质谱尿液泛酸生物监测

Keywords

liquid chromatography tandem mass spectrometryurinepantothenic acidbiomonitoring

references

Views

704

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the Method for the Identification of Sulfanilamide，Quinolone and Tetracycline in Urine by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

Detection of 12 Novel Psychoactive Substances of Benzodiazepines in Urine by Direct Analysis of Real Time－Tandem Mass Spectrometry

Simultaneous Determination of 10 2C-series Phenethylamines in Blood and Urine by UPLC－MS/MS

Simultaneous Determination of Nicotine and Its Nine Metabolites in Human Urine Using Hydrophilic Interaction High Performance Liquid Chromatography－Tandem Mass Spectrometry

Related Author

No data

Related Institution

Institute of Nutrition and Health，Chinese Center for Disease Control and Prevention

Beijing Center for Disease Control and Prevention，Beijing Center for Preventive Medicine Research

Forensic Science Service of Beijing Public Security Bureau，Key Laboratory of Forensic Toxicol，Ministry of Public Security

School of Investigation，People’s Public Security University of China

School of Criminal Investigation，People’s Public Security University of China

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰