A nano-silver/poly quercetin composites modified paraffin-impregnated graphite electrode was prepared by electrochemical method(Ag/Qu/WGE).A mixture of L-Thr,chitosan and Nafion was coated on the surface of Ag/Qu/WGE,L Thr(template molecule) was removed by electrochemical cleaning using potentiostatic method.As a result,a molecular imprinted polymer modified electrode based on chitosan/nano-silver/poly quercetin composites could be obtained for L-Thr detection(TMIP/WGE).The characteristics of the modified electrode were investigated by means of field emission scanning electron microscope technique,X-ray photoelectron spectroscopy,infrared spectra and electrochemical technique.The result indicated that the TMIP/WGE displayed a good electro-catalysis towards the oxidation of L-Thr,which could be used for sensitive and quick detection of L-Thr.The differences of oxidation peak current of L-Thr(1.45 V) showed a good linearity in the concentration range of 0.1-1.0 μmol/L with a detection limit(3σ) of 0.01 μmol/L.The electrode was successfully applied in the determination of L-Thr in fermentation broth.