Preparation and Electrochemical Analysis Application of Enzyme-functionalized Prussia Blue-Polydopamine-Platinum Nanocomposites

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Preparation and Electrochemical Analysis Application of Enzyme-functionalized Prussia Blue-Polydopamine-Platinum Nanocomposites

- Preparation and Electrochemical Analysis Application of Enzyme-functionalized Prussia Blue-Polydopamine-Platinum Nanocomposites
- Vol. 35, Issue 10, Pages: 1289-1294(2016)
- 作者机构：
  
  1. 石河子大学化学化工学院新疆兵团材料化工工程技术研究中心
  2. 新疆维吾尔自治区教育厅高校材料化工重点实验室
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Preparation and Electrochemical Analysis Application of Enzyme-functionalized Prussia Blue-Polydopamine-Platinum Nanocomposites. [J]. 35(10):1289-1294(2016)
DOI：

Preparation and Electrochemical Analysis Application of Enzyme-functionalized Prussia Blue-Polydopamine-Platinum Nanocomposites. [J]. 35(10):1289-1294(2016) DOI：

摘要

在纳米金表面原位沉积普鲁士蓝，然后在核壳结构纳米金-普鲁士蓝的表面包覆一层易氧化聚合的多巴胺保护膜，利用多巴胺聚合表面残留的大量氨基和羟基进一步将纳米铂粒子修饰于聚多巴胺膜表面制得普鲁士蓝-聚多巴胺-纳米铂多层纳米复合材料。将此复合材料修饰于金电极表面，协同使用辣根过氧化物酶用于H2O2浓度的检测。结果表明：聚多巴胺的引入有效增加了普鲁士蓝的稳定性，增大了纳米铂的负载量以及辣根过氧化物酶的生物活性；由于普鲁士蓝、纳米铂和辣根过氧化物酶的多重信号放大作用，酶功能化纳米复合材料修饰电极对H2O2表现出良好的电还原活性。优化条件下，对H2O2的检测范围为2.0×10-7~1.0×10-3 mol?L-1，检出限(S/N=3)为1.2×10-7 mol?L-1。

Abstract

In this paper，prussian blue(PB) coated gold nanoparticles(Au NPs) were firstly prepared by in situ method.Then，polydopamine(PDA) wrapped Au-PB was synthesized based on the characteristic of one-step oxidative polymerization of dopamine.The bioinspired surface enriched amino and hydroxyl groups were further used as a support to anchor active platinum nanoparticles(Pt NPs).Horseradish peroxidase(HRP) functionalized Au-PB-PDA-Pt nanocomposites was also used for the fabrication of H2O2 biosensor.By taking advantages of the excellent biocompatibility and film forming ability of PDA，the large surface area and high biocompatibility of Pt NPs，and the synergistic employ of PB，Pt and HRP，the HRP functionalized nanocomposites modified electrode exhibited an excellent electroreduction activity to H2O2.Under the optimum conditions，the linear detection range from 2.0×10-7-1.0×10-3 mol?L-1 was observed,and a detection limit was 1.2×10-7 mol?L-1.

关键词

过氧化氢传感器纳米复合材料普鲁士蓝聚多巴胺辣根过氧化物酶

Keywords

H2O2 sensornanocompositesprussia bluepolydopaminehorseradish peroxidase

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Related Institution

Key Lab of Resource Chemistry＆Eco-environmental Protection in Qinghai－Tibet Plateau， State Ethnic Affairs Committee，Qinghai Minzu University

College of Chemistry and Chemical Engineering，Qinghai Minzu University

Jiaxing Key Laboratory of Molecular Recognition and Sensing，College of Biological，Chemical Science and Engineering， Jiaxing University

College of Science and Life Sciences，Zhejiang Normal University

中南大学化学化工学院

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