A cell capture scaffold with a structure of Subs-PAA1-SiO2-PAA2-Apt was assembled by using a glass slide as substrate carrier(Subs),polyelectrolyte polyacrylic acid(PAA) as cross-linking agent and flexible body,SiO2 nanoparticles as rigid body,and TD05 aptamer(Apt) as capture ligand of objective cell Ramos.Here there are a small scale and rigid structure(i.e.SiO2 nanoparticle),but also a flexible structure(i.e.polyacrylic acid PAA1 and PAA2).The former increased the roughness of carrier,and the latter could not only immobilized the multi-ligand Apt,but also decreased the steric hindrance from the scaffold itself and the carrier for cell capture.The results indicated that the as-assembled scaffold had a good nanoparticle structure and fusing even disappearing of the nanoparticle structure was not found.For the scaffold,its specific capture was 16 times of the non-specific adsorption,and the captured cell showed a higher purity.Meanwhile the captured cell has also a higher captureefficiency,and the capture cell number was 8.3 times of that of the usual rigid scaffold with one-layer nanoparticle.It was shown that the nanoparticle flexible scaffold with a novel structure was fabricated,which could realize the high purity and the high efficiency for cell capture.