A new method was developed for the rapid determination of diclazuril，toltrazuril，toltrazurilsulfone and toltrazurilsulfoxide residues in foods of animal origin by on line cleanup liquid chromatography-high resolution benchtop quadropole/obitrap mass spectrometry（HRMS） .The samples were extracted with acetonitrile，and purified on a Cyclone column.The analytes were eluted from the exaction column and put into a C18 analytical column prior to chromatographic separation and detection in full scan/ddMS2 mode.The calibration curves were linear in the range of 0.5-50 ng/mL with correlation coefficients more than 0.999 4.The limits of quantitation（LOQ） were 2 μg/kg.Average recoveries of the analytes in four matrices ranged from 80.2% to 110.5%，with relative standard deviations of 2.8%-9.7%.With simple pretreatment，low matrix effects,this method could be used for the rapid confirmation with accurate mass of quasi molecular ion，retention time，isotope ratio and qualitative fragments.