A method based on ultrasonic assisted dispersive emulsion microextraction procedure coupled to high performance liquid chromatography with fluorescence detector(UA-DLLME-HPLC-FLD) was developed for the determination of nine fluorescent whitening agents (FWAs) residues in skincare water.Experimental conditions affecting the microextraction procedure such as type and volume of extraction solvent，content of disperse solvent，salt concentration，pH value，extraction time and centrifugal time were investigated.The ultrasonic assisted disperse emulsion microextraction conditions were optimized by using 50 μL chloroform as solvent and 250 μL methanol as disperser solvent，and the mixture was centrifuged at 4 500 r/min for 2 min.Under the optimum conditions，the calibration curves of nine fluorescent whitening agents in a certain concentration range with correlation coefficients more than 0.999 0.The limits of detection(LODs) of night fluorescent whitening agents ranged from 0.06 mg/kg to 4.20 mg/kg and the limits of quantitation(LOQs) ranged from 0.2 mg/kg to 14.0 mg/kg.The average recoveries for nine fluorescent whitening agents residues in skincare water were in the range of 84.3%-102.4%.Compared to the previous methods，the new procedure has the characteristics of simple sample preparation，low organic solvent consumption and high sensitivity.