To eliminate the interference on the determination of selenium（Se） in royal jelly by inductively coupled plasma mass spectrometry（ICP-MS），a dynamic reaction cell（DRC） method was established by the study of reaction gas species，flow rate and isotope of Se.The results showed that 78Se was suitable for the analysis.The interference on the determination of Se was eliminated when CH4 was used as reaction gas and its flow rate was set to 0.5 mL?min-1.The method had a wide linear range of 0.1-100 μg?L-1 and a low detection limit of 0.052 2 μg?L-1.The relative standard deviations（RSD） were less than 5%.The recoveries were between 95.0% and 103.9%.The differences of the results of Se in royal jelly samples determined by DRC-ICP-MS and atomic fluorescence spectrometry were not significant.