An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the determination of 14 quinolones in hotpot.Samples were extracted with acidified acetonitrile,defated with n hexane,separated on a C18 solid phase extraction(SPE) column,and analyzed by LC-ESI-MS/MS under the positive mode with multiple reaction monitoring(MRM).The method showed good linearities for 14 quinolones over the range of 7.5-100 μg/L with correlation coefficients(r) more than 0.990.The detection limits of 14 quinolones were 0.5 μg/kg,and the quantitation limits were 1.5 μg/kg.The recoveries of all analytes in hotpot ranged from 75.4% to 110.0%with relative standard deviations(RSD,n=6)of 1.3%-10.1%.The method has the advantages of simplicity,rapidness,good purifying effect and sensitivity,and is suitable for the simultaneous determination of quinolones in hotpot.