A method was developed for the rapid detection of 8 hormones，6 β-receptor agonists and 4 antibiotics growth promoting agents in bovine liver by PRiME HLB solid-phase extraction/ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The chromatographic separation conditions，MS/MS detection parameters and sample pretreatment were optimized，respectively.The sample was firstly added NH4OAc buffer solution(pH 5.2)，and hydrolyzed with a mixed solution of β-glucuronidase/sulfatase，then extracted with methanol(MeOH) and acetonitrile(ACN)-MeOH(90∶10，by volume) step by step，finally purified with PRiME HLB.The effluent was collected and concentrated to dryness，and redissolved with ACN-water(3∶7，by volume) for UPLC-MS/MS detection.The results showed that 18 kinds of compounds had good linear relationships in the range of 1.0-100.0 μg/L with their correlation coefficients higher than 0.990.The detection limits(LODs) ranged from 0.07-0.78 μg/kg，and the quantitation limits(LOQs) ranged from 0.23-2.58 μg/kg.The average recoveries of 18 compounds at three spiked levels(0.5，1.0，5.0 μg/kg) were in the range of 67.5%-102.0%with relative standard deviations(RSDs) less than 15%.The method was simple，rapid and accurate，and was suitable for the simultaneous detection of 18 growth promoting agents in animal tissues.