A novel electrochemical sensor for the determination of ribavirin(RIB) was fabricated by molecularly imprinted technology，based on 3-aminobenzeneboronic acid as functional monomer，and ribavirin as target molecule.A molecularly imprinted polymers（MIPs） membrane was synthesized on the surface of glassy carbon electrode in vacuum drying oven，by the formation principle of ring ester bond with boric acid and vicinal diol.The electrochemical behavior of the membrane was characterized by cyclic voltammetry(CV) and differential pulse voltammetry(DPV).Under the optimum conditions，the response of peak currents was linear to concentration of RIB in the range of 5.0×10-8-1.0×10-5 mol/L with a detection limit(S/N=3) of 1.5×10-8 mol/L.The electrochemical sensor has the advantages of high sensitivity，good stability and high reproducibility，and could be uesd to detect RIB in food.