A new method for the determination of vancomycin and norvancomycin residues in shrimps by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) was established.Samples were extracted with 0.1% formic acid-acetonitrile solution,defatted with n hexane saturated with acetonitrile and purified with PCX and Florisil solid phase extraction column.The separation of two targets was carried out on a CAPCELL PAK MG-C18 column by gradient elution with 0.1% formic acid-acetonitrile as mobile phase．The identification of two analytes was performed in multi-reaction monitoring(MRM),and the quantification was carried out by the internal standard method.Dedichloro vancomycin was used as an internal standard.The calibration curves showed good linearities in the range of 2-250 ng/mL with correlation coefficients(r2) greater than 0.99.The detection limits(LOD) of this method were both 2.0 μg/kg,and the limits of quantitation(LOQ) were 5.0 μg/kg.The recoveries of the target compounds at spiked levels of 5,25,50 μg/kg were in the range of 87.2%-102%,with relative standard deviations(RSDs) of 1.3%-8.7%.The results indicated that this method had high sensitivity,accuracy and good reproducibility.It was suitable for the simultaneous determination of ancomycin and norvancomycin residues in shrimps.