An ultra-performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the determination of thirteen sex hormones in cosmetics.Toner,oil,cream and mask cosmetic samples were extracted with 50% acetonitrile by vortex,and separated on a C18 column．The analysis was performed under dynamics multiple reaction monitoring(DMRM) mode.The results showed that the limits of detection(LODs) for 13 sex hormones in various kinds of cosmetics were in the range of 0.001-0.026 μg/g.Good linear relationship existed between peak area and mass concentration of 0.89-64.80 μg/L with correlation coefficients higher than 0.995.The average recoveries were in the range of 80.8%-116.8%,with relative standard deviations(RSDs,n=6) of 0.7%-14.4%.With the advantages of high efficiency,sensitivity and accuracy,this method fills the gaps of determining chlormadinone acetate and other sex hormones,and is suitable for the general detection of neutral sex hormones in cosmetics.