An HPLC-ECD method was established for the determination of five neurotransmitters in cerebral tissues of rats to discuss the protective effects of alltride(DAS) on the monoamine neurotransmitters(MNTs) in paraquat(PQ) poisoning rats brain tissues. Male ICR rats were divided into four groups(n=6) according to their different treatments. The paraquat(PQ) group and the treatment(PQ+DAS) group exposed to 35mg/kg PQ i.g.. The control group and the alltride(DAS) group were given the same volume of sterile saline. The alltride(DAS) group and treatment group were administrated with 25mg/kg DAS i.p. after PQ was given intragastrically for 2,26,50 h.PQ group and normal group rats were injected with the same amount of sterile saline. The determination was performed on an Acclaim 120 C18 column with a mobile phase consisted of buffer salt(sodium citrate,monopotassium phosphate,sodium heptanesulfonate,EDTA-2Na,diethylamine hydrochloride )-methanol(93∶7,by volume) at a flow rate of 0.27mL/min,and the voltage of electrode was set at 450mV.Compared with the normal or DAS group，the PQ group showed significant decreases in the quantities of DA and 5-HT.The combination of PQ and DAS resulted in higher amounts of neurotransmitters than that of the administration with PQ alone(P<0.05).The spiked recoveries were in the range of 79.8%-112.1% with RSDs lower than 10%.DAS could prevent rats from the PQ-induced decrease of the neurotransmitters, which may be associated with its abilities to relieve oxidative damages.