A rapid method was developed for the screening and quantification of 28 pesticides endocrine disruptors in aquatic products by ultrahigh performance liquid chromatography-quadrupole/electrostatic field obitrap high-resolution mass spectrometry.The samples were extracted with ethyl acetate,and purified with a Captiva ND Lipids column.The separation was carried out on an ACQUITY UPLC@BEN C18 column(2.1 mm×50 mm,1.7 μm) with methanol-water as mobile phases by gradient elution.The 28 pesticides endocrine disruptors were simultaneously analyzed in switching positive and negative modes.The retention time,accurate mass of parent ion and relative isotope abundance were used for fast screening in full scan mode,while accurate mass of fragment ions were used for comfirmation in two-stage threshold-triggered full mass scan mode.Under the optimal conditions,good linearities(r2>0.99) were obtained in their respective linear ranges.The limits of detections(LODs) for most compounds were not more than 10 μg/kg except for acetochlor,procymidone and fenitrothion.The recoveries were in the range of 65.7%-98.9% and the relative standard deviations(RSDs) were 6.2%-11.3%.The method was simple,sensitive and accurate,and was suitable for the rapid screening and confirmation of multiple pesticides endocrine disruptors in aquatic products.