A method with high sensitivity,high throughout and low sample consumption was developed for the analysis of trace elements in human serum by inductively coupled plasma mass spectrometry(ICP-MS).As the matrix was complicated,the sample was diluted with a mixture of four butyl ammonium hydroxide,Triton and EDTA.The internal standard correlation and collision/reaction cell were used to eliminate the matrix effects and polyatomic ion inteferences.Ten trace elements of Al,Cu,Fe,Zn,Ga,As,Cd,Hg,Tl and Pb were simultaneously determined after tuning up the instrument and optimizing the collision/reacion cell parameters,and the spiked recovery for each element ranged from 87.2% to 111.2%.This method was used in the analysis of human serum standard(ClinChek-Control，Level Ⅰ and Level Ⅱ).The determined values were in good agreement with the certified values.The reference values for different age groups and sex groups were listed based on the determination of 127 serum samples from healthy adults.