An ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the determination of 13 anti-diabetic ingredients illegally added in formulated foods on special medical purposes.Samples were extracted with 90% acetonitrile containing 1% formic acid,and then purified with an Oasis PRiME HLB column.The extraction was on a Waters ACQUITY UPLC BEH C18(150 mm×2.1 mm，1.7 μm) column with methanol-5 mmol/L ammonium acetate solution containing 0.1% formic acid as mobile phases by gradient elution.The analysis on the analyte was performed by UPLC-MS/MS under positive electrospray ionization in multiple reaction monitoring(MRM) mode,Effects affecting the recoveries such as dosage of extractant,formic acid and acetonitrile in cleaning-up agent were investigated.The main influence factors and analytical conditions were modified.Results showed that there were good linear relationships for 13 ingredients in the concentration range of 2-100 μg/L with their correlation coefficients(r2) no less than 0.995 1.The recoveries at three concentration levels ranged from 80.9% to 102.8% with LOQs of 0.3-1.0 μg/kg.With simple pretreatment,high recovery and good reproducibility,the developed method was suitable for the determination of 13 anti-diabetic ingredients illegally added in formulated foods on special medical purposes.