An analytical method based on ultra performance liquid chromatography-quadrupole linear ion trap mass spectrometry(UPLC-QTRAP-MS/MS) was established for the simultaneous determination of galanthamine(Gal),lycoramine(Lycm) and lycorine(Lyc) in Lycoris.After ultrasonic-assisted extraction and centrifugation,the sample was seperated on a Waters ACQUITY UPLC BEH C18 column(150 mm×2.1 mm，1.7 μm) by gradient elution with acetonitrile-water(0.1% formic acid) as mobile phase at a flow rate of 0.2 mL/min.The three target compounds were analyzed in the positive ion multiple reaction monitoring(MRM) mode.Results showed that good linearities for the analytes were obtained in the concentration range of 0.5-500 μg/L with their correlation coefficients of 0.999 9-1.000 0 within 6 mins,and the retention times for Gal,Lycm and Lyc were 2.86 min,2.31 min and 1.95 min,respectively.The average recoveries were between 97.2%-98.6% with relative standard deviations of 0.8%-4.3%. The contents of Gal,Lycm and Lyc in 12 Lycoris species were determined to be 21.98-496.77 μg/g,0.17-467.21 μg/g and 9.34-4 510.18 μg/g by dry weight,respectively.Large differences were observed among species.This method was sensitive and accurate,and could provide a reliable and effective approach for the accumulation detection and metabolic regulation of alkaloids in Lycoris．