A method for the determination of advantame in foods using high performance liquid chromatography（HPLC） was developed.The samples were extracted with methanol-water（50∶50,by volume） in ultrasonic condition for 10 min.The supernatant was analyzed after centrifugation.Target compound was separated on an Agilent TC-C18（2）（250 mm×46 mm,5 μm） column at 280 nm,with methanol-002 mol/L ammonium acetate（53∶47,by volume） as mobile phase by isocratic elution.The quantitative analysis was carried out by the external standard method.The results showed that the calibration curves were linear in the range of 10-2000 mg/L with correlation coefficients of 0999 8.The limits of detection（S/N≥3） of the method for the analytes in beverage,yoghourt and cake were 06，15 and 90 mg/kg,respectively.The average recoveries ranged from 909% to 1034% with relative standard deviations（RSDs,n=5） of 11%-45%.With the advantages of good separation,few impurity disturbances and high accuracy,this method could be applied in the analysis of advantame in real samples.