A method was established for the determination of chiral difenoconazole in dragon fruit by solid phase extraction coupled with high performance liquid chromatography.The dragon fruit samples were extracted with acetonitrile，and purified with carbon black/amino solid phase extraction column.Effects of different chiral columns，mobile phase compositions，flow rates and injection volumes on chiral separation were investigated.The optimized chromatographic conditions were as follows：the chromatographic column：IF-3 chiral column，mobile phase：n hexane-ethanol（92∶8 by volume），injection volume：60 μL，flow rate：10 mL/min.Under the optimized conditions，the four difenoconazole enantiomers were basically achieved baseline separation，and the analysis time was less than 35 min.There existed a good linearity for each enantiomer in the range of 010-10 mg/L，with a detection limit of 0025 mg/L.The average recoveries for the chiral difenoconazole in dragon fruit at two spiked levels of 04 mg/kg and 28 mg/kg were in the ranges of 837%-993% and 864%-916%，with their RSDs of 13%-72% and 14%-31%，respectively.The developed method is rapid，accurate and sensitive，and is applicable for the determination of chiral difenoconazole residues in dragon fruit，etc.