In the absence of ion-pair reagents or high-salt buffer condition，a novel method for the quantitative analysis of disodium ethylenediaminotetraacetic acid in flour and its products by high performance liquid chromatography was developed，employing hydrophilic C18 reversed-phase liquid chromatography column and methanol-water system as mobile phase.Samples were extracted with aqueous solution，and reacted with ferric chloride by ultrasound irradiation to product stable derivative which was cleaned up with trichloromethane.To determine ethylenediaminotetraacetic acid disodium in flour and flour products，the analytes were determined by high performance liquid chromatography with a wavelength of 260 nm and a hydrophilic C18 reversed phase liquid chromatography column using mobile phases of pH 4.0 methanol and aqueous solution(10∶90，by volume).Under the optimal conditions，the calibration curves of the analytes were linear in the concentration range of 1.0-50.0 mg/L with the correlation coefficients of 0.999 6.The limits of quantification(LOQs) were 10.0 mg/kg，and the limits of detection(LODs) were 3.0 mg/kg.At spiked levels of 10.0-200 mg/kg，the average recoveries of spiked samples ranged from 95.0%to 103%，with relative standard deviations between 4.0%and 6.3%.The method could meet the requirement for the rapid screening and quantitative analysis of disodium ethylenediaminotetraacetic acid in flour and its products.