A method was developed for the determination of 19 quinolone antibacterials residues in aquatic products by high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with PRiME HLB purification technique.In this research,the parameters such as purification,concentration,liquid phase separation and mass spectrometry were optimized.The samples were extracted with 80%acetonitrile-water mixed solution,purified with a PRiME HLB column,and quantified by UPLC-MS/MS in MRM mode after gradient elution on an ACQUITY BEH C18 column(1.7 μm,2.1 mm×100 mm) with methanol and 5 mmol/L ammonium acetate solution containing 0.1%formic acid as mobile phase.The results showed that there existed good linear relationships for the components in their respective concentration ranges.The average recoveries were in the range of 72.1%-119.9%with the relative standard deviations of 2.4%-15.6%，the detection limits of 0.5 μg/kg and the quantitation limits of 1.5 μg/kg.With the advantages of good accuracy,simplicity and high sensitivity,this method is suitable for the determination of quinolone antibacterials in aquatic products,and it provides a new way for the determination of quinolone antibacterials residues in aquatic products.