Digital microfluidics（DMF）is an emerging liquid handling technique based on arrays of microelectrodes for precise control of discrete droplets.This platform based on electrowetting on dielectric（EWOD）has the characteristics of automation,addressability,flexible device configuration,and easy combination with other techniques compared with the conventional microchannel based fluidics.The mechanism for droplet actuation,the structure and fabrication of microfluidic chips and their applications in the field of bio analytical chemistry are presented in this paper.And an outlook for the future applications of DMF is also proposed.