A high performance liquid chromatography(HPLC) method combined with a rapid and simple pretreatment approach was established for the determination of five fluoroquinolones(ciprofloxacin,danofloxacin,enrofloxacin,difloxacinand and sarafloxacin) residues in eggs.Samples were extracted with acetonitrile.Protein in egg samples was precipitated by heating in water-bath.The supernatants were degreased with n hexane,and followed by HPLC analysis.The mobile phase consisted of 005 mol/L phosphoric acid and triethylamine-acetonitrile(80∶20).The fluorometric excitation wavelength was 280 nm,and the emission wavelength was 450 nm.The limits of quantitation(LOQ) for ciprofloxacin,difloxacin,danofloxacin,enrofloxacin and sarafloxacin were 5,5,05,25 and 10 μg/kg,respectively.The average recoveries for 5 fluoroquinolones were in the range of 922%-1071% at four spiked levels(LOQ,2 LOQ,4 LOQ and 20 LOQ),and the intra day and inter day relative standard deviations(RSDs) were in the ranges of 06%-58% and 15%-50%,respectively.The proposed method is rapid,accurate,sensitive and eco friendly,and could be used for the quick analysis on residues of fluoroquinolones in egg in its production and distribution for consumption.