A method based on high performance liquid chromatography（HPLC） with online monolithic column solid phase extraction was developed for the determination of diflubenzuron and hexa flumuron in fruits. The polymer（poly（2 ethylhexyl methacrylate co ethylene dimethacrylate）） monolithic column（10 mm×46 mm，id.） was prepared by using 2 ethylhexyl methacrylate（EHMA） as monomer and ethylene dimethacrylate（EDMA） as crosslinker，which exhibited a porous structure with a specific surface area of 16401 m2·g-1. Using polymer monolithic column as online solid phase extraction sorbent，the extracted fruit sample solution was loaded into the monolithic column using 5 mmol·L-1 NH4OAc at a flow rate of 01 mL·min-1. The adsorbed analytes were transferred from monolithic column to the analytical column using acetonitrile-water（65∶35，by volume） as mobile phase at a flow rate of 10 mL·min-1. Under the optimized conditions，there was a good linearty for the method in the range of 01-500 mg·L-1（r≥0998）. The limits of detection and quantitation for diflubenzuron and hexaflumuron were 0007 2 mg·kg-1 and 0009 mg·kg-1，and 0024 mg·kg-1 and 0030 mg·kg-1，respectively. The recoveries at three spiked levels of 003，03 and 15 mg·kg-1 were in the range of 895%-102% with intra day and inter day relative standard deviations not more than 68%.