In order to achieve the selective adsorption of heavy metal ions in environmental aqueous solution，a thiol groups functionalized gold nanoparticles metal stirring bar（AuNPs-SH-SBSE） was fabricated by self assembling 1,8 octanedithiol on surface of the etched stainless steel deposited with gold nanoparticles（AuNPs）.The extraction and separation properties of the stirring bar were evaluated with Pb（Ⅱ） and Cd（Ⅱ） by ICP-OES.Effects of adsorption time，pH value and desorption solvent on the adsorption were investigated.The results showed that，using equilibrium time of 30 min and an optimum pH of 80，60 mL 15 mol/L HNO3 as eluate，the maximal adsorption capacity of the stirring bar toward Pb（Ⅱ） and Cd（Ⅱ） reached to 985% and 874%，respectively.Under the optimal conditions，the method was successfully applied in the determination of trace Pb（Ⅱ） and Cd（Ⅱ） in real samples.There existed linearities for Pb（Ⅱ） and Cd（Ⅱ） in the ranges of 01-50 mg/L and 02-20 mg/L，with their limits of detection（S/N=3） of 24 ng/L and 36 μg/L，respectively.The recoveries at two spiked levels of 20 μg/L and 50 μg/L were in the ranges of 854%-1050% and 742%-978%，with their RSDs（n=3） of 38%-82% and 42%-106%，respectively.The method was simple，rapid and sensitive，and could be applied in the separation and detection of Pb（Ⅱ） and Cd（Ⅱ） in environmental water.