Protein glycosylation is one of the most important post translational modifications.However,the great challenges for glycosylation of protein/peptides are the inherently low abundance and the poor ionization efficiency in mass spectrometry identification.Therefore,the specific enrichment before MS identification is indispensable.The basic principles,characteristics and recent progresses of the enrichment approaches for glycosylated proteins/peptides are reviewed,and the development prospects of the enrichment approaches for N glycosylated proteins/peptides are also outlined.