An analytical method was established for the simultaneous screening and determination of 112 prohibited drugs,including quinolones,glucocorticoids,nitroimidazoles,tetracyclines,macrolides,sulfonamides,cephems and androgens in cosmetics by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The sample was extracted with 0.2% formic acid-acetonitrile,and then purified by QuEChERS method.The extracts were separated on an Agilent ZorbaxEclipsePlus C18(3.0 mm×150 mm,1.8 μm) column with 0.2% formic acid-acetonitrile solution containing 0.2% formic acid as mobile phase by gradient elution,and analyzed by LC-MS/MS under dynamic multiple reaction monitoring(DMRM) mode.Results showed that the limits of quantitation(LOQ,S/N=10) for 112 target compounds in cosmetics were in the range of 2.0-10.0 μg/kg.The mean recoveries for 112 prohibited drugs were in the range of 82.0%-108% with their RSDs of 2.0%-7.9%.The method has the advantages of simplicity,rapidness and high efficiency,and could be applied in the screening and confirmation on 112 prohibited drugs in cosmetics in routine analysis.