A novel method was developed for the detection of HbAlc using a screen printed electrode with 3-aminophenylboronic acid modified magnetic nanoparticles.The magnetic nanoparticles modified with 3-aminophenylboronic acid were prepared,which could bind with glyeosylated hemoglobin(HbA1c) and be separated from hemoglobin when the sample is injected into the reticulated vitreous carbon(RVC) flow cell.A screen printed electrode was fabricated by the reaction of chitosan(CS),tetraenthoxyl silica(TEOS) and carbon nanotubes to form a sol-gel layer at the surface of the electrode.Based on this,a novel electrochemical method was developed for the detection of HbA1c.There were good relationships of electric charge at the screen printed electrode with concentrations of HbA1c and Hb under the potential of 0.10 V, and the limits of detection were 6 mg/mL and 0.05 mg/mL，respectively.With simple preparation,good reproducibility and stability,the method could effectively eliminate the interference of AA and UA.It was successfully applied in the detection of HbA1c in real samples.